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A Rapid Method for Distinguishing D-Glucosamine from Galactosamine in Biological Preparations

Milton W. Slein

Abstract

An enzymatic method is described for distinguishing D-glucosamine from galactosamine. The method is based on the fact that glucosamine is phosphorylated by ATP in the presence of hexokinase, whereas galactosamine is not. Application of the procedure for the assay of biological preparations is illustrated by the demonstration of D-glucosamine in hydrolysates of a polysaccharide isolated from Shigella flexneri.

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Key concepts: Glucosamine, Galactosamine, Shigella flexneri, Hexokinase, Chemistry, Biochemistry, Hydrolysate, Enzyme