2000•Biomedical Optical Spectroscopy and DiagnosticsRequires access
A new method to simultaneously determine the absorption and elastic scattering spectra of tissues in vivo
Philippe Thueler, Frédéric Bevilacqua, Christian Depeursinge, Gaëlic Ory, Nadereh Azar-Pey, Bernard Vermeulen, Igor Charvet, Domenico Bosco, Paolo Meda
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Abstract
Different techniques have been proposed to correlate optical properties of tissue and their physiology/pathology. In this paper, we describe a new optical biopsy method based on spatially resolved reflectance measurement using a small diameter probe (ø1.8 mm). The development of an appropriate model for photon propagation in tissue at such distances allows us to determine simultaneously reduced scattering coefficient µs’ and absorption coefficient µa. Being made spectroscopically, the measurements then provide information both on tissue structure (related to scattering coefficient) and chromophore content (related to absorption coefficient). Such features can be used to distinguish between normal tissues and various pathologies. We present the guidelines of the method and preliminary results obtained on mice, where significant contrast has been observed between normal and altered epidermis and dermis.