The Flow of Fluid in the Cornea

Trypan blue and victoria blue 4R were introduced into the stroma of the cornea of adult rabbits and the following results were gained.1. In the peripheral part of the cornea of tha rabbit trypan blue, which is inserted, spreads mainly toward the center. But in the central part trypan blue spreads equally in all directions. And its reason was examined.2. Trypan blue, which is insoluble in lipid, spreads in the stroma of the cornea and stains the membrane of DESCEMET, but it cannot enter the epithelium, which is located on the anterior surface of the cornea, and endothel, which is located on the posterior surface of the cornea. Victoria blue 4R, which is insoluble in water, but soluble in lipid, can hardly spread in the stroma, but enter the epithelium relatively well. If seems that these differences are due mainly to the different amounts of lipids which are contained in the various layers of the cornea.