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Construction of a .BETA.-glucanase hyperproducing Bacillus subtilis using the cloned .BETA.-glucanase gene and a multi-copy plasmid.

Hidetoshi Tezuka, Toshifumi Yuuki, Seizo Yabuuchi

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Abstract

Wehave cloned the /?-glucanase gene from a /f-glucanase producing strain, Bacillus subtilis Y-25, to construct a /?-glucanase hyperproducing strain.The cloned 1.9 Kb EcoRl-Hpal fragment containing the entire /?-glucanase gene was inserted into the EcoRl and Pvull sites of a multi-copy vector plasmid, pUBllO.The resulting plasmid, named pLBlOO,was introduced into B. subtilis Y-25 to construct B. subtilis HL-25.HL-25 produced 347 units/ml of /?-glucanase in a culture supernatant, which was about 19-fold higher than the amount produced by the original strain, Y-25, and corresponded to

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Wehave cloned the /?-glucanase gene from a /f-glucanase producing strain, Bacillus subtilis Y-25, to construct a /?-glucanase hyperproducing strain.The cloned 1.9 Kb EcoRl-Hpal fragment containing the entire /?-glucanase gene was inserted into the EcoRl and Pvull sites of a multi-copy vector plasmid, pUBllO.The resulting plasmid, named pLBlOO,was introduced into B. subtilis Y-25 to construct B. subtilis HL-25.HL-25 produced 347 units/ml of /?-glucanase in a culture supernatant, which was about 19-fold higher than the amount produced by the original strain, Y-25, and corresponded to

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Available abstract

Wehave cloned the /?-glucanase gene from a /f-glucanase producing strain, Bacillus subtilis Y-25, to construct a /?-glucanase hyperproducing strain.The cloned 1.9 Kb EcoRl-Hpal fragment containing the entire /?-glucanase gene was inserted into the EcoRl and Pvull sites of a multi-copy vector plasmid, pUBllO.The resulting plasmid, named pLBlOO,was introduced into B. subtilis Y-25 to construct B. subtilis HL-25.HL-25 produced 347 units/ml of /?-glucanase in a culture supernatant, which was about 19-fold higher than the amount produced by the original strain, Y-25, and corresponded to

Key concepts: Glucanase, EcoRI, Bacillus subtilis, Plasmid, Bacillaceae, Gene, Recombinant DNA, Chemistry

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Construction of a .BETA.-glucanase hyperproducing Bacillus subtilis using the cloned .BETA.-glucanase gene and a multi-copy plasmid. — Research Paper | ScholarLens