Tetrazolium Stains for Diphosphopyridine Nucleotide (DPN) Diaphorase and Triphosphopyridine Nucleotide (TPN) Diaphorase in Animal Tissue.
Emmanuel Farber, William H. Sternberg, Charles E. Dunlap
Abstract
Emmanuel Farber, William H. Sternberg, Charles E. Dunlap
Abstract
Methods for the specific staining for DPN diaphorase and TPN diaphorase in frozen sections of fresh tissue are presented. The staining patterns of rat kidney are highly reproducible and are distinctly different for each of the 2 diaphorases and for the succinic dehydrogenase system.
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Methods for the specific staining for DPN diaphorase and TPN diaphorase in frozen sections of fresh tissue are presented. The staining patterns of rat kidney are highly reproducible and are distinctly different for each of the 2 diaphorases and for the succinic dehydrogenase system.
Key concepts: Diaphorase, Succinic dehydrogenase, Staining, Biochemistry, Formazan, Chemistry, Nucleotide, Cofactor