1987•Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)Open access

Enumeration of Lactic Acid Bacteria in Fermented Milks and Lactic Acid Bacteria Drinks by the Plate MPN Technique

Shingo Mizuochi, Keiko Yoshida, Hirokazu Ogihara, Misao HARUTA

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Abstract

In 1983, Tan and his co-workers presented a plate MPN technique for estimating viable cell counts as a substitute for the traditional method. This simple system was compared with the pour plate method for the estimation of lactic acid bacterial counts in fermented milks and lactic acid bacteria drinks.The correlation coefficients between plate MPN counts and pour plate counts in pure cultures of lactic acid bacteria, and fermented milks and lactic acid bacteria drinks were 0.983 and 0.929, respectively.The plate MPN technique appears to be a convenient procedure for the estimation of lactic acid bacterial counts in fermented milks and lactic acid bacteria drinks.

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In 1983, Tan and his co-workers presented a plate MPN technique for estimating viable cell counts as a substitute for the traditional method. This simple system was compared with the pour plate method for the estimation of lactic acid bacterial counts in fermented milks and lactic acid bacteria drinks.The correlation coefficients between plate MPN counts and pour plate counts in pure cultures of lactic acid bacteria, and fermented milks and lactic acid bacteria drinks were 0.983 and 0.929, respectively.The plate MPN technique appears to be a convenient procedure for the estimation of lactic acid bacterial counts in fermented milks and lactic acid bacteria drinks.

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Available abstract

In 1983, Tan and his co-workers presented a plate MPN technique for estimating viable cell counts as a substitute for the traditional method. This simple system was compared with the pour plate method for the estimation of lactic acid bacterial counts in fermented milks and lactic acid bacteria drinks.The correlation coefficients between plate MPN counts and pour plate counts in pure cultures of lactic acid bacteria, and fermented milks and lactic acid bacteria drinks were 0.983 and 0.929, respectively.The plate MPN technique appears to be a convenient procedure for the estimation of lactic acid bacterial counts in fermented milks and lactic acid bacteria drinks.

Key concepts: Lactic acid, Bacteria, Fermentation, Food science, Enumeration, Plate count, Microbiology, Biology

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