1989•Laboratory MedicineRequires access
Sulfation-Hematoxylin-Azure B as an Alternative to Periodic Acid-Schiff Staining
Stanley H. Shapiro
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Abstract
Gentle sulfation of formalin-fixed tissue sections followed by staining in a hematoxylin-azure B sequence results in bright blue staining of tissue elements that stain with the periodic acid-Schiff reaction—especially basement membranes. The stain is relatively rapid, simple, and permanent, is performed with inexpensive, readily available laboratory reagents, and is presented asan alternative to periodic acid-Schiff staining.