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2009•Unpublished venueRequires access

In Vitro Properties of MEDI-4212, a Human Anti-IgE Antibody for the Treatment of Allergic Asthma.

E. Suzanne Cohen, P.O. Eriksson, Karin A. von Wachenfeldt, Isabelle de Mendez, Dorothy A. Sims, Sarah J Oakley, C.L. Dobson, Sofia Persdotter, Helena Ekdahl, Maryam Azimi, Ulf Alkner, Phillip Monk, Scott L. Klakamp, S OBrian, Clare M. Lloyd, Mats Carlsson, Anthony J. Coyle, Ian Anderson

Open publisher page 4 citations

Abstract

HuFceRI transfected RBL or LAD2 cells were incubated with IgE ± MEDI4212. Bound IgE was cross-linked using a commercial anti-IgE and cell activation detected using calcium signalling or β-hexosaminidase release. Effect of MEDI4212 on IgE/CD23 interaction was assessed by incubation of IL-4 stimulated IM9 cells with IgE ± MEDI4212 and bound IgE detected by flow cytometry. Effects on IgE production in human B cells (differentiated with APC + IL-4, ± MEDI4212) was evaluated by flow cytometry. Serum free-IgE was measured using ELISA.

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What this paper is about

HuFceRI transfected RBL or LAD2 cells were incubated with IgE ± MEDI4212. Bound IgE was cross-linked using a commercial anti-IgE and cell activation detected using calcium signalling or β-hexosaminidase release. Effect of MEDI4212 on IgE/CD23 interaction was assessed by incubation of IL-4 stimulated IM9 cells with IgE ± MEDI4212 and bound IgE detected by flow cytometry. Effects on IgE production in human B cells (differentiated with APC + IL-4, ± MEDI4212) was evaluated by flow cytometry. Serum free-IgE was measured using ELISA.

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Available abstract

HuFceRI transfected RBL or LAD2 cells were incubated with IgE ± MEDI4212. Bound IgE was cross-linked using a commercial anti-IgE and cell activation detected using calcium signalling or β-hexosaminidase release. Effect of MEDI4212 on IgE/CD23 interaction was assessed by incubation of IL-4 stimulated IM9 cells with IgE ± MEDI4212 and bound IgE detected by flow cytometry. Effects on IgE production in human B cells (differentiated with APC + IL-4, ± MEDI4212) was evaluated by flow cytometry. Serum free-IgE was measured using ELISA.

Key concepts: Immunoglobulin E, CD23, Flow cytometry, Chemistry, Antibody, Immunology, In vitro, Basophil

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In Vitro Properties of MEDI-4212, a Human Anti-IgE Antibody for the Treatment of Allergic Asthma. — Research Paper | ScholarLens