ScholarLens Back to search
2016•Cold Spring Harbor ProtocolsRequires access

Transformation of Schizosaccharomyces pombe: Electroporation Procedure

Johanne M. Murray, Adam T. Watson, Antony M. Carr

Open publisher page 12 citations

Abstract

Transformation ofSchizosaccharomyces pombewith DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. This protocol describes transformation by electroporation. It involves pretreatingS. pombecells with dithiothreitol (DTT), which increases the transformation efficiency once the electric pulse is applied.

About this research paper

What this paper is about

Transformation ofSchizosaccharomyces pombewith DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. This protocol describes transformation by electroporation. It involves pretreatingS. pombecells with dithiothreitol (DTT), which increases the transformation efficiency once the electric pulse is applied.

Why it matters

OpenAlex reports 12 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Transformation ofSchizosaccharomyces pombewith DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. This protocol describes transformation by electroporation. It involves pretreatingS. pombecells with dithiothreitol (DTT), which increases the transformation efficiency once the electric pulse is applied.

Key concepts: Electroporation, Transformation (genetics), Protoplast, Plasmid, Transformation efficiency, Schizosaccharomyces pombe, Dithiothreitol, DNA

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Transformation of Schizosaccharomyces pombe: Electroporation Procedure — Research Paper | ScholarLens