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Comparison Between Free Amino Acid Levels in Plasma Deproteinated with Picric Acid and with Sulfosalicylic Acid.

Wolfram Block, Mara E. Markovs, Betty F. Steele

Abstract

A comparative study of 20% sulfosalicylic acid and of 1% picric acid in precipitating plasma proteins before analysis for free amino acids by ion-exchange chro-matography showed that both methods give values comparable in precision and accuracy. The sulfosalicylic acid method is less time-consuming, and requires fewer manipulations.

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Key concepts: Sulfosalicylic acid, Picric acid, Chemistry, Amino acid, Chromatography, Biochemistry, Organic chemistry