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1989•Journal of Nutritional Science and VitaminologyOpen access

Determination of vitamin B6 derivatives in foods and biological materials by reversed-phase HPLC.

Toshikatsu Toukairin-Oda, Eiko Sakamoto, Naoyuki Hirose, Mitsuaki Mori, Takeshi Itoh, Haruhito Tsuge

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Abstract

Through use of a simplified analyzing system, seven vitamin B6 derivatives were determined with a satisfactory sensitivity and precision. This system consisted of a single reversed-phase ODS column with a fluorescence detector employing an isocratic solvent system. Each vitamin B6 derivative in some foods and biological materials was determined, based on the measurement of the integrated peak area. The data obtained by this method were compared with those obtained from a bioassay by Saccharomyces uvarum ATCC 9080, after acid hydrolysis of these materials.

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Through use of a simplified analyzing system, seven vitamin B6 derivatives were determined with a satisfactory sensitivity and precision. This system consisted of a single reversed-phase ODS column with a fluorescence detector employing an isocratic solvent system. Each vitamin B6 derivative in some foods and biological materials was determined, based on the measurement of the integrated peak area. The data obtained by this method were compared with those obtained from a bioassay by Saccharomyces uvarum ATCC 9080, after acid hydrolysis of these materials.

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Available abstract

Through use of a simplified analyzing system, seven vitamin B6 derivatives were determined with a satisfactory sensitivity and precision. This system consisted of a single reversed-phase ODS column with a fluorescence detector employing an isocratic solvent system. Each vitamin B6 derivative in some foods and biological materials was determined, based on the measurement of the integrated peak area. The data obtained by this method were compared with those obtained from a bioassay by Saccharomyces uvarum ATCC 9080, after acid hydrolysis of these materials.

Key concepts: Chromatography, Bioassay, Vitamin b6, Vitamin, Chemistry, High-performance liquid chromatography, Solvent, Hydrolysis

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Determination of vitamin B6 derivatives in foods and biological materials by reversed-phase HPLC. — Research Paper | ScholarLens