Prolongation of Hexobarbital Narcosis and Zoxazolamine Paralysis in Mice Pretreated with Food Additives

Hexobarbital sleeping time (HST) and zoxazolamine paralysis time (ZPT) were measured in male mice after oral administration of 14 food additives. HST and ZPT were significantly (P<0.05) prolonged when hexobarbital or zoxazolamine was given intraperitoneally after pretreatment with diphenyl, o-phenylphenol, piperonyl butoxide or thiabendazole. One hour after the pretreatment, the maximum prolongation of HST and ZPT was observed.To prolong HST or ZPT in mice, it was necessary to administer 50mg/kg of diphenyl, o-phenylphenol or thiabendazole to them. However, a dose of only 5mg/kg of piperonyl butoxide prolonged HST and ZPT.