Immunoassays in forensic sciences.
Kimiko Aoki, Yukio Kuroiwa
Abstract
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Kimiko Aoki, Yukio Kuroiwa
Abstract
Open-access reader
There are various drugs to be analysed in the forensic science. Of all analytical methods, an immunoassay is suitable to detect some drugs in biological materials because of its high sensitivity and simple pretreatment. This review has been presented on the method for the preparation of immunogen, principles of non-isotopic immunoassays, and immunoassays as the screening method with microtiter plate and/or latex particles. The examples of hapten synthesis were the drug causing dependence and pesticides. The immunoassay will be a more powerful tool in using monoclonal antibody and developing a simple and rapid analytical system.
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There are various drugs to be analysed in the forensic science. Of all analytical methods, an immunoassay is suitable to detect some drugs in biological materials because of its high sensitivity and simple pretreatment. This review has been presented on the method for the preparation of immunogen, principles of non-isotopic immunoassays, and immunoassays as the screening method with microtiter plate and/or latex particles. The examples of hapten synthesis were the drug causing dependence and pesticides. The immunoassay will be a more powerful tool in using monoclonal antibody and developing a simple and rapid analytical system.
Key concepts: Immunoassay, Immunogen, Hapten, Chromatography, Microtiter plate, Monoclonal antibody, Chemistry, Biology