Mini and Microsatellite DNA Analyses of the Irradiated Sperm Transformed Chickens.

We analysed our irradiated sperm transformed (IST) chicken DNA for minisatellite loci by DNA fingerprinting and the microsatellite DNA by PCR method.The direct coordination between brownish egg shell color and DNA fingerprint bands was not recognized under this study condition. The genetic similarities of WL-γ 5 (IST line), WLG (recipient line) and RIRY8 (donor line) by using band sharing (BS) values were calculated from the number of DNA fingerprint bands derived from mixed DNA. The value of BS between WL-γ5 and WLG was 0.61 in HaeIII digestion bands and 0.48 in HinfI digestion bands. The BS value between WL-γ5 and RIRY8 was 0.26 in HaeIII digestion bands and 0.15 in HinfI digestion bands. Comparison of the DNA fingerprint of mixed DNA for each strain was made and the common band in both WL-γ5 and RIRY8 was detected. This band was not detected in WLG. Strain specific band was detected from DNA fingerprint used in mixed DNA. The major band was common in WL-γ5 and WLG. In PCR analysis, the common band in both WL-γ5 and RIRY8 was found. From these results, it was thought that the WL-γ5 genome was, for the most part, common with the recipient WLG genome, except for a few genome from RIRY8 donor DNA. We thought that a few parts of donor DNA in WL-γ5 were transferred through the irradiated sperm of donor RIRY8 male under successive selections for the brownish egg shell color from generation to generation.