Identification and Isolation of a Wild Strain of Infectious Bursal Disease Virus

笔者从北京延庆地区一鸡场采集发病鸡的肾脏、脾、法氏囊样品，通过鸡胚接种及RT-PCR技术初步诊断其病原为传染性法氏囊病毒，暂命名为BY-2015-1。本文设计IBDV VP2的引物，以反转录得到的cDNA为模板进行扩增，并对扩增得到的序列进行序列分析，序列分析显示该分离株的核苷酸序列与标准强毒株OKYM的同源性为97.7%，分析VP2高变区的氨基酸序列发现，此分离株在VP2高变区关键氨基酸位点的变异符合强毒株的特点，但又不完全相同。该毒株的分离和鉴定，为传染性法氏囊病防控和流行病学调查提供了依据。 The kidney, spleen and bursa of fabricius samples were collected from diseased chickens on a farm in Beijing suburban areas. A wild strain of infectious bursal disease virus (IBDV), temporarily called BY-2015-1, was isolated by embryonic inoculation and identified via RT-PCR assay and sequence analysis. Gene sequence analysis of VP2 showed that the homology between BY-2015-1 and OKYM strains was 97.7%. The amino acid sequence analysis of VP2 high variable region indicated that BY-2015-1 isolate had similar characteristics of very virulent strains, suggesting that the isolate might be a circulating virulent strain in the flocks on the farm. The isolation and identification of IBDV BY-2015-1 strain would help to prevent and control IBDV in the local areas.