BACILLARY DYSENTERY IN CYNOMOLGUS MONKEYS FOLLOWING THE ADMINISTRATION WITH SHIGELLA FLEXNERI 2A BY THE ANAL ROUTE

This method, however, is more or less disadvantageous for quantitative analyses, since it is uncertain whether all the orally administered bacilli can reach the large intestine, the primary site of Shigella infection.Direct injection of a Shigella flexneri 2 a suspension into the cecal lumen proved useful to evoke clinical manifestation of dysentery even with a relatively small dose such as 107 or 105 bacilli, which could not elicit pronounced symptoms of dysentery by oral administration (Takasaka et al., 1968).This method, however, involves some difficulties not only in performing surgical operation on many monkeys at one time but also in explaining the obtained results that might be affected by the possible modification of susceptibility of the animals by the surgical operation.The method of infection with Shigella described in this paper may overcome such experimental difficulties and may give reliable results with relatively small number of animals.