Culture of Mycelia from the Fruit Body of Hymenomycetes by ^|^ldquo;The Hymenium-Isolation Method^|^rdquo;

(1) Experiments on nineteen species of hymenomycetous fungi showed that the best part in isolating the mycelium from the fruit body is that of hymenium.(2) When this “Hymenium-Isolation Method” was applied to the hymenomycetous fungi which grow on the forest land, a suitable culture medium was pine-needle decoction agar.(3) It is often impossible to use the part of pileus flesh or stipe tissue for isolating the mycelium, for being small or often damaged by insects. In such cases, a desirable result can be obtained by applying “The Hymenium-Isolation Method”.(4) There are not a few cases where hyphae do not grow out from the part of pileus flesh or stipe; and even if the hyphae grow out, they are scanty in number and their isolation is sometimes difficult. However, when the part of hymenium is used, a luxuriant growth of hyphae can often be seen.(5) In adopting “The Hymenium-Isolation Method”. the best result can be obtained when the gill is used. When the needle or the tube is used, it is sometimes difficult to isolate the mycelium because of bacterial contamination. Nevertheless the needle or the tube is superior to the part of pileus or stipe in a same fruit body.