Demonstration of Treponema-like Organisms by Direct Fluorescein Antibody Technique in Lymphnodes from Treated Late Human and Experimental Early Syphilis

There were demonstrated Treponema-like organisms by direct fluorescein antibody technique in the popliteal lymphnodes from 4 cases of treated early experimental syphilis with long lasting reactive VDRL slide test and in the human inguinal lymphnodes from 5 of late syphilis, 3 of congenital and one of secondary (probably reinfektion) syphilis after treatment. This Treponema-like organisms were identified with Treponema pallidum from morphological and immunofluorescein technical findings. Moreover, these persistence and long durated reagin levels after treatment were seemed to indicate their vital maintenance. Persistence of these organisms in the lymphnodes or other organ were attributed to the continued persistence of antibodies on the treatment-resistant syphilis. These material lymphnodes were inoculated into normal rabbits intratestically, in order to study the virulence of those organisms, neverthless, there were not found the syphilitic orchitis and the clear increasing of anitbodies.