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2015•Journal of Evolution of Medical and Dental SciencesOpen access

COMPARATIVE EVALUATION OF VARIOUS LABORATORY M ETHODOLOGIES IN THE DIAGNOSIS OF MALARIA

R. Hymavathi, J. Vijayalakshmi, Swarnalatha Guditi

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Abstract

BACKGROUND: Malaria is the most important protozoan disease in India.The mortality and morbidity associated with this disease is considerably high whereas only limited diagnostic modalities are available in India.AIMS: To compare the conventional peripheral smear examination with Quantitative buffy coat (QBC) and rapid antigen detection kits.MATERIALS & METHODS: In the present study a total of 200 patients with clinically suspected malaria are screened with various techniques including conventional peripheral smear examinations with Giemsa, Leishman and JSB stain and newer modalities like Quantitative buffy coat testing and rapid antigen detection kits detecting PLDH.RESULTS: Among all these methodologies QBC test has shown highest sensitivity and antigen detection kit showed least sensitivity in the diagnosis of malaria.

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BACKGROUND: Malaria is the most important protozoan disease in India.The mortality and morbidity associated with this disease is considerably high whereas only limited diagnostic modalities are available in India.AIMS: To compare the conventional peripheral smear examination with Quantitative buffy coat (QBC) and rapid antigen detection kits.MATERIALS & METHODS: In the present study a total of 200 patients with clinically suspected malaria are screened with various techniques including conventional peripheral smear examinations with Giemsa, Leishman and JSB stain and newer modalities like Quantitative buffy coat testing and rapid antigen detection kits detecting PLDH.RESULTS: Among all these methodologies QBC test has shown highest sensitivity and antigen detection kit showed least sensitivity in the diagnosis of malaria.

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Available abstract

BACKGROUND: Malaria is the most important protozoan disease in India.The mortality and morbidity associated with this disease is considerably high whereas only limited diagnostic modalities are available in India.AIMS: To compare the conventional peripheral smear examination with Quantitative buffy coat (QBC) and rapid antigen detection kits.MATERIALS & METHODS: In the present study a total of 200 patients with clinically suspected malaria are screened with various techniques including conventional peripheral smear examinations with Giemsa, Leishman and JSB stain and newer modalities like Quantitative buffy coat testing and rapid antigen detection kits detecting PLDH.RESULTS: Among all these methodologies QBC test has shown highest sensitivity and antigen detection kit showed least sensitivity in the diagnosis of malaria.

Key concepts: Medicine, Malaria, Diagnosis of malaria, Intensive care medicine, Immunology, Plasmodium falciparum

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