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1989•Nippon Nōgeikagaku KaishiOpen access

Hydrolysis of stevioside with baker's yeast and Rh. japonicus.

Tadamasa Terai

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Abstract

Stevioside (1), natural sweet tasteic diterpenoid, was hydrolyzed with baker's yeast (peptone, yeast extract, malt extract medium; 30°C, 7 days) to produce a high yield of steviol (2). The hydrolysis of stevioside with Rh. japonicus gave steviolbioside (4) as the main product. Quantitative analysis of stevioside and its derivatives was performed by thin layer chromatography. The optimum conditions were: developing solvent, CH•Cl3: MeOH: H2O=30:15:2 or CHCl3:MeOH=93:7; detection agent, 20% phosphotungstic acid: 10% phosphomolybdic acid=1:1; TLC plate, Merck Fertigplatten Kieselgel 60.

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Stevioside (1), natural sweet tasteic diterpenoid, was hydrolyzed with baker's yeast (peptone, yeast extract, malt extract medium; 30°C, 7 days) to produce a high yield of steviol (2). The hydrolysis of stevioside with Rh. japonicus gave steviolbioside (4) as the main product. Quantitative analysis of stevioside and its derivatives was performed by thin layer chromatography. The optimum conditions were: developing solvent, CH•Cl3: MeOH: H2O=30:15:2 or CHCl3:MeOH=93:7; detection agent, 20% phosphotungstic acid: 10% phosphomolybdic acid=1:1; TLC plate, Merck Fertigplatten Kieselgel 60.

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Available abstract

Stevioside (1), natural sweet tasteic diterpenoid, was hydrolyzed with baker's yeast (peptone, yeast extract, malt extract medium; 30°C, 7 days) to produce a high yield of steviol (2). The hydrolysis of stevioside with Rh. japonicus gave steviolbioside (4) as the main product. Quantitative analysis of stevioside and its derivatives was performed by thin layer chromatography. The optimum conditions were: developing solvent, CH•Cl3: MeOH: H2O=30:15:2 or CHCl3:MeOH=93:7; detection agent, 20% phosphotungstic acid: 10% phosphomolybdic acid=1:1; TLC plate, Merck Fertigplatten Kieselgel 60.

Key concepts: Stevioside, Chemistry, Hydrolysis, Steviol, Yeast, Chromatography, Acid hydrolysis, Thin-layer chromatography

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