PHARMACODYNAMIC INTERACTION OF QUERCETIN AND SILYMARIN AGAINST CYCLOPHOSPHAMIDE INDUCED CARDIO-TOXICITY

The study was designed to investigate the cardioprotective effect of different combination of Silymarin and Quercetin against Cyclophosphamide induced cardiotoxicity in experimental rats.The Albino rats of either sex were divided into five groups of six animals.Group 1 and group 2 served as normal and control group respectively.Other groups treated with Silymarin (60 mg/kg, p.o.) alone, high (30 mg/kg, p.o.) and low (15 mg/kg, p.o.) dose of Silymarin combined with Quercetin (10 mg/kg, p.o.).Myocardial damage was induced by the administration of Cyclophosphamide (200mg/kg, i.p.) on the first day and the treatment was conducted for ten days.Twenty-four hours after the last administration, the blood was collected by the retro-orbital puncture method and the serum biomarker level of Lactate Dehydrogenase (LDH), Creatinine Kinase-NAC (CK-NAC), Creatinine Kinase-MB (CK-MB), Aspartate Transaminase (AST), Alanine Transaminase (ALT) and Alkaline Phosphatase (ALP) in serum were evaluated.Electro-cardiograph parameters such as Heart rate, QRS interval, QT segment, PR interval and RR interval also carried out.Compared to the control group all treated group showed significant activity and high and low dose combination group showed profound cardiac protection compare to the Silymarin alone.Among the entire groups high dose combination group (SILLY30+QUE10) showed most effective Cardio-protection and was also responsible for the significant improvement in the ECG parameters.