Hematology of the Leopard Frog, Rana pipiens

A study of the hematology of Rana pipiens including blood cell counts, blood volume, pH, hematocrit, hemoglobin, coagulation time and fragility was made. Red blood cell counts varied from 120,000 to 470,000 (mean 319,400); white cell counts 2000-9800 (mean 5500) and thrombocytes 350015200 (mean 7300) per mm3. The white cell differential count was also made. Blood counts were found to be highly variable and it was not possible to determine any seasonal influence in the total count. The pH of the blood was between 7.2 and 7.6 (mean 7.36), hematocrit reading 1339% (mean 24.65), hemoglobin 2.4-9.6 g/100 ml (mean 6.75), coagulation time 60-90 seconds (mean 70), and fragility 0.175-0.250% of NaC1 (mean 0.215). The description of the various types of blood cells and blood cell photographs are given for the first time for R. pipiens. l of the Leopard Frog, Rana pipiens