ScholarLens Back to search
1966•Biochemical JournalOpen access

The deoxyribonucleic acid of Micrococcus radiodurans

AH Schein

Open full text 10 citations

Abstract

The DNA of Micrococcus radiodurans was prepared by three methods. Although the recovery of DNA varied considerably, the percentage molar base ratios of the DNA from the three preparations were essentially the same: guanine, 33+/-2; adenine, 18+/-1; cytosine, 33+/-2; thymine, 17+/-1. Base compositions calculated from T(m) values and from density in caesium chloride gradients also yielded guanine+cytosine contents of 66 and 68% of total bases respectively. No unusual bases were observed. The S(20,w) values were characteristic of high-molecular-weight DNA. Electron microscopy showed the purified DNA in long strands; occasionally these were coiled.

Open-access reader

About this research paper

What this paper is about

The DNA of Micrococcus radiodurans was prepared by three methods. Although the recovery of DNA varied considerably, the percentage molar base ratios of the DNA from the three preparations were essentially the same: guanine, 33+/-2; adenine, 18+/-1; cytosine, 33+/-2; thymine, 17+/-1. Base compositions calculated from T(m) values and from density in caesium chloride gradients also yielded guanine+cytosine contents of 66 and 68% of total bases respectively. No unusual bases were observed. The S(20,w) values were characteristic of high-molecular-weight DNA. Electron microscopy showed the purified DNA in long strands; occasionally these were coiled.

Why it matters

OpenAlex reports 10 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The DNA of Micrococcus radiodurans was prepared by three methods. Although the recovery of DNA varied considerably, the percentage molar base ratios of the DNA from the three preparations were essentially the same: guanine, 33+/-2; adenine, 18+/-1; cytosine, 33+/-2; thymine, 17+/-1. Base compositions calculated from T(m) values and from density in caesium chloride gradients also yielded guanine+cytosine contents of 66 and 68% of total bases respectively. No unusual bases were observed. The S(20,w) values were characteristic of high-molecular-weight DNA. Electron microscopy showed the purified DNA in long strands; occasionally these were coiled.

Key concepts: Cytosine, Guanine, Thymine, DNA, Micrococcus, Chemistry, Nucleic acid, Base (topology)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
The deoxyribonucleic acid of Micrococcus radiodurans — Research Paper | ScholarLens