Measurement of the Mechanical Deformation of Organ of Corti in a Model of Acute Endolymphatic Hydrops

Background and ObjectivesZZEndolymphatic hydrops has been considered as an important histologic substrate of Meniere's disease.A permanent displacement of basilar membrane (BM) by increased endolymphatic pressure has been thought to be an explanation for hearing change.Direct observation of histological sections of temporal bones, however, suggested that stereocilia and tectorial membrane decoupling is more associated with pressure induced by mechanical deformation of the organ of Corti rather than with the displacement of BM.Meterials and MethodZZ26 cochleae from 13 female pigmented ginea pigs were harvested.One cochlea per each animal was injected with artificial perilymph.The other one was used as control.After fixation, followed by embedding and mid-modiolar sectionning, specimens were observed with a microscope.Morphometric parameters of each row and turn of the organ of Corti were measured and quantified.ResultsZZThe average area and height of the organ of Corti were significantly smaller in the apical turn of the experimental group (p＜0.05).The lengths of outer hair cell and Deiters cell in the apical turn were also significantly reduced in the experimental group (p＜0.05).The angle between the outer hair cell and Deiters cell was smaller in the apex and in the 3rd turn of the experimental group (p＜0.05).ConclusionZZResults show that compression and deformation of the organ of Corti, especially in the apical turn, is a prominent feature in the acute endolymphatic hydrops model.We suggest that the deformation of organ of Corti is the primary cause of hydrops that induce the decoupling of tectorial membrane and stereocilia rather than the displacement of BM.