In vitro free radical scavenging potential of methanol extract of entire plant of Phyllanthus reticulatus poir

Summary The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values were 14.31 µgm/ml (DPPH radical scavenging), 10.51 µgm/ml (ABTS radical scavenging), 22.91µgm/ml (O-phenanthroline assay), 32.16 µgm/ml (lipid peroxidation), 90.51µgm/ml (nitric oxide scavenging) and 4.18µgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31µg of ascorbic acid. It showed 83.69% inhibition of haemoglobin glycosylation.