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2014•African Journal of Microbiology ResearchOpen access

Recovery of lipophilic Malassezia species from two infants with otitis media in Monterrey, Nuevo Len, Mexico

Diana Elisa Zamora-Ávila, Guerra-Lund Rafael, Juan Antonio Vidales-Contreras, Patricia Da Silva-Nascente, Marlete Brum-Cleff, Jesús Jaime Hernández-Escareño

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Abstract

Malassezia species are yeasts that can be present on healthy human skin but also associated with external otitis and other skin diseases. The aim of this work was the identification of Malassezia -lipophilic- species recovered from infants with otitis media. Microbiological culture, polymerase chain reaction (PCR) and sequencing were performed for Malassezia detection in samples obtained from two cases of infants with otitis media. We identified two strains of lipid-dependent Malassezia species: M. furfur and M. restricta. This is the first report regarding the presence of Malassezia species in infants with otitis media in Monterrey, México. Key words: Malassezia, Malassezia furfur, Malassezia restricta, otitis media, infants, LSU rDNA D1/D2, yeasts.

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Malassezia species are yeasts that can be present on healthy human skin but also associated with external otitis and other skin diseases. The aim of this work was the identification of Malassezia -lipophilic- species recovered from infants with otitis media. Microbiological culture, polymerase chain reaction (PCR) and sequencing were performed for Malassezia detection in samples obtained from two cases of infants with otitis media. We identified two strains of lipid-dependent Malassezia species: M. furfur and M. restricta. This is the first report regarding the presence of Malassezia species in infants with otitis media in Monterrey, México. Key words: Malassezia, Malassezia furfur, Malassezia restricta, otitis media, infants, LSU rDNA D1/D2, yeasts.

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Available abstract

Malassezia species are yeasts that can be present on healthy human skin but also associated with external otitis and other skin diseases. The aim of this work was the identification of Malassezia -lipophilic- species recovered from infants with otitis media. Microbiological culture, polymerase chain reaction (PCR) and sequencing were performed for Malassezia detection in samples obtained from two cases of infants with otitis media. We identified two strains of lipid-dependent Malassezia species: M. furfur and M. restricta. This is the first report regarding the presence of Malassezia species in infants with otitis media in Monterrey, México. Key words: Malassezia, Malassezia furfur, Malassezia restricta, otitis media, infants, LSU rDNA D1/D2, yeasts.

Key concepts: Malassezia, Otitis, Microbiology, Dermatology, Medicine, Biology, Surgery

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Recovery of lipophilic Malassezia species from two infants with otitis media in Monterrey, Nuevo Len, Mexico — Research Paper | ScholarLens