Cytological study of male sterility and pollenabortion in two types of grapes

Male sterility of grape cultivars ‘2004-6-12’(Wink seedling),‘2004-2-30’(Kyoho seedling)and Wink(CK) and their anther features were examined. The pollen viability, pollen shape and interior structure were investigated as well. The results indicated that the pollen viability of ‘2004-6-12’and ‘2004-2-30’were 0. The pollen grain characteristics differed in ornamentation, especially in type of germinal aperture and shape. The pollen grains of ‘2004-6-12’ and ‘2004-2-30’ did not have any germinal furrow and were approximately spheroidal (‘2004-6-12’: P/E=1.13±0.05,‘2004-2-30’: P/E=1.12±0.04),while that of Wink was tricolporate and prolate spheroidal (P/E=2.11±0.08). The edges of pollen grain cross-section in both ‘2004-6-12’and‘2004-2-30’were glazed. The pollen ornamentation of Wink was foveolate, but that of ‘2004-6-12’ and ‘2004-2-30’were reticulate and cavernous, respectively. The cytological features of anther and microspore development in ‘2004-6-12’, ‘2004-2-30’ and Wink were examined by a light microscope. The microspores of ‘2004-6-12’and‘2004-2-30’mostly disintegrated at the early mononuclear microspore stage; the anther of ‘2004-6-12’ partly began to be abnormal at the meiosis stage, but the anther of ‘2004-2-30’was normal before the early mononuclear microspores stage. The disintegration of microspores of ‘2004-6-12’and‘2004-2-30’were correlated with the earlier disintegration of tapetum.