1969•Cold Spring Harbor Symposia on Quantitative BiologyRequires access
A DNA-Directed Cell-Free System for -Galactosidase Synthesis; Characterization of the De Novo Synthesized Enzyme and Some Aspects of the Regulation of Synthesis
Geoffrey Zubay, Donald A. Chambers
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Abstract
Many aspects of the regulation of cellular RNA and protein synthesis could best be studied in a DNA-directed cell-free system. Toward this aim, our laboratory is attempting to reconstruct, in a cell-free system, the events which take place in the gene-directed synthesis of the enzyme fl-galactosi-dase in E. coli. The genetic region of E. coli con-taining the fl-galaetosidase gone, termed the lae operon, is surrounded by a family of related genes as indicated in Fig. 1. Starting from the left, they consist of (1) a regulator gene, i, which encodes the lac repressor protein; (2) a promoter locus required for expression of the lac operon; (3) an operator locus required for the i-gene-induced repression of the lac operon and; (4) z, y, and a, the structural