2014•Monoclonal Antibodies in Immunodiagnosis and ImmunotherapyRequires access
Generation of a Monoclonal Antibody Against the Extracellular Domain of IGF-1R
Yusuke Nakadate, Saori Yoshimura, Naoko Nakatani, Masayuki Azuma, Taro Tachibana
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Abstract
Type I insulin-like growth factor receptor (IGF-1R) is a receptor tyrosine kinase that is involved in the transformation of cells, cancer proliferation, and metastatic events in various types of cancer. The present study reports on the generation of a mouse monoclonal antibody (MAb) to IGF-1R using the mouse lymph node method. MAb 1B1, which reacts specifically with the extracellular domain of IGF-1R, was obtained using flow cytometry (FCM) screening using MCF-7 cells. Using immunostaining, MAb 1B1 detected IGF-1R mainly on the plasma membrane of MCF-7 cells. MAb 1B1 would be useful in FCM and immunofluorescence assays to detect IGF-1R-expressing cells for basic and clinical research.