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2006•Japanese Journal of Oral & Maxillofacial SurgeryOpen access

Analysis of platelet aggregation and TGF-.BETA. content in platelet-rich plasma

Sanae Watatani, Megumi YANAGIDA, Hiroaki Suzuki, Satoshi Yokoo, Takahide Komori

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Abstract

Recently, platelet-rich plasma (PRP) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of PRP from small quantities of blood.Various conditions were used for anticoagulants and centrifugal separation speed for blood samples obtained from 23 healthy subjects to investigate optimal concentration conditions. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r. p. m. and 1300 r.p.m. The effectiveness of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after double centrifugation.

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What this paper is about

Recently, platelet-rich plasma (PRP) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of PRP from small quantities of blood.Various conditions were used for anticoagulants and centrifugal separation speed for blood samples obtained from 23 healthy subjects to investigate optimal concentration conditions. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r. p. m. and 1300 r.p.m. The effectiveness of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after double centrifugation.

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Available abstract

Recently, platelet-rich plasma (PRP) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of PRP from small quantities of blood.Various conditions were used for anticoagulants and centrifugal separation speed for blood samples obtained from 23 healthy subjects to investigate optimal concentration conditions. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r. p. m. and 1300 r.p.m. The effectiveness of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after double centrifugation.

Key concepts: Platelet-rich plasma, Centrifugation, Platelet, Medicine, Platelet aggregation, Platelet concentrate, Chromatography, Andrology

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