High-sensitive flow injection analysis of proteins coupled with on-line alkaline hydrolysis and the fluorescent derivatization method.
Hideaki Ichiba, Hiroshi NIWAYAMA, Takehiko Yajima
Abstract
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Hideaki Ichiba, Hiroshi NIWAYAMA, Takehiko Yajima
Abstract
Open-access reader
Sub-nanogram levels of proteins were determined by a flow-injection analysis coupled with an on-line alkaline hydrolysis and o-phthalaldehyde fluorescent derivatization method.
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Sub-nanogram levels of proteins were determined by a flow-injection analysis coupled with an on-line alkaline hydrolysis and o-phthalaldehyde fluorescent derivatization method.
Key concepts: Derivatization, Chemistry, Flow injection analysis, O-Phthalaldehyde, Chromatography, Alkaline hydrolysis, Fluorescence, Hydrolysis