[Patch-clamp technique].
Norihiko Akaike, T Shirasaki
Abstract
Norihiko Akaike, T Shirasaki
Abstract
We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of conventional patch-clamp techniques except the cell-attached mode.
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We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of conventional patch-clamp techniques except the cell-attached mode.
Key concepts: Clamp, Patch clamp, Rectification, Materials science, Computer science, Engineering, Electrical engineering, Electrophysiology