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[Patch-clamp technique].

Norihiko Akaike, T Shirasaki

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Abstract

We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of conventional patch-clamp techniques except the cell-attached mode.

About this research paper

What this paper is about

We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of conventional patch-clamp techniques except the cell-attached mode.

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Available abstract

We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of conventional patch-clamp techniques except the cell-attached mode.

Key concepts: Clamp, Patch clamp, Rectification, Materials science, Computer science, Engineering, Electrical engineering, Electrophysiology

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