Chromatographic Demonstration of Histamine Release in Anaphylactic and Trypsin Shock

A method for the chemical identification of histamine in blood by paper chromatography is described in detail and experiments are presented to show the release of relatively large amounts of histamine in dogs undergoing anaphylactic and trypsin shock. Peptone, when injected into dogs in amounts sufficient to produce severe shock, interfered with the identification of histamine which could, however, be found on the chromatograms after addition of small amounts of peptone to rabbit blood (in vitro).