GW24-e1903 Effect of ox-LDL and atorvastatin on the production of interleukin-18 in and the proliferation of cultured human umbilical vein endothelial cells

Objectives To investigate the effects of atorvastatin on the production of interleukin-18(IL18) in and the proliferation of cultured human umbilical vein endothelial cells (HUVECs) activated by oxidised low density lipoprotein (Ox-LDL). Methods HUVECs Cultured in vitro at passage 3 to 9 were used for experiment. There were three groups: 1) control group; 2) Ox-LDL groups (100 mg/L); 3) Ox-LDL + atorvastatin groups: cells were firstly incubated with 0.01, 0.05, 0.1, 0.5, 1 µmol/L atorvastatin respectively for 4 h,then incubated with 100 mg/L Ox-LDL for 24 h. IL18 in supernatant medium was detected by cell ELISA and cell proliferation was measured by MTT colorimetric assay. Results Compared with control group,ox-LDL (100 mg/L) could inhibit the proliferation of HUVECs (P < 0.01). Atorvastatin could dose-dependently enhance the proliferation of HUVECs activated by Ox-LDL (P < 0.05, P < 0.01). HUVECs didn’t release IL18 without stimulation. ox-LDL(100 mg/L) stimulated the production of IL18 by HUVECs. Atorvastatin (0.05, 0.1, 0.5, 1 µmol/L) could inhibit the production of IL18 in HUVECs activated by Ox-LDL (P < 0.05, P < 0.01), but not in 0.01 µmol/L (P < 0.05). The inhibitory effect was dose-dependent. Conclusions atorvastatin can obviously inhibited the production of IL18 in HUVECs and enhanced the proliferation of HUVECs activated by Ox-LDL, which might improve the impaired endothelial cell beyond lipid regulation function.