Assay of Serum Inorganic Phosphate without Deproteinisation: Automated and Manual Micromethods

Several current techniques for the determination of serum inorganic phosphate in the presence of proteins are shown to have important disadvantages. An improved manual micromethod is described in which proteins are kept in solution by the use of sodium lauryl sulphate and the phosphate determined by the molybdenum blue reaction. The procedure has been adapted for the Technicon AutoAnalyzer and the Vickers D-300 and M-300, and has been shown to give very good precision in routine use.