1950•Experimental Biology and MedicineRequires access

Biuret Reaction as Applied to Determination of Antibody Nitrogen

Morris Solotorovsky, C. C. Porter, R Silber

Abstract

A quantitative biuret method for the determination of protein has been applied to the measurement of antibody N. The nitrogen contents of duplicate antigen-antibody precipitates from rabbit anti-bovine albumin serum and rabbit anti-egg albumin sera, plus the homologous antigens, were determined by the biuret and micro-Kjeldahl methods. The results agreed within ± 0.02 mg nitrogen with precipitates containing 0.1 to 0.5 mg antibody nitrogen.

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Key concepts: Biuret test, Kjeldahl method, Antibody, Chemistry, Nitrogen, Bovine serum albumin, Antigen, Chromatography