Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma

Chinese Journal of CancerEsophageal squamous cell carcinoma (ESCC) is a common gastrointestinal cancer with poor diagnosis due to metastasis.It has been found that Ezrin interacts with Ecadherin and plays a role in tumor cell metastasis.The present study detected the expressions of Ezrin and Ecadherin in ESCC using tissue array with immunohistochemistry, and analyzed their relations with malignancy degree and metastasis of ESCC, so as to provide new evidence for the diagnosis and prognosis prediction of ESCC.A tissue array consisting of 76 specimens of ESCC was prepared.The 76 patients, including 49 men and 27 women, underwent operation between July 2004 and July 2007 in Department of Thoracic Surgery of the Affiliated Hospital of Hebei University of Engineering.The median age was 62.5 years.None received radiotherapy or chemotherapy before operation.The diagnosis of ESCC was confirmed in all patients by postoperative pathologic examination.Of the 76 cases, 26 were well differentiated, 33 were moderately differentiated and 17 were poorly differentiated; 12 were at stage T1, 21 at stage T2, 29 at stage T3 and 14 at stage T4; 41 had lymphatic metastasis while 35 did not.Pathologic sections were prepared and HE staining was performed.One specimen of cancer tissue and one specimen of paracancer normal tissue (35 cm from the cancer tissue) were obtained from each case to prepare 10 mm 伊 8 mm arrays with a thickness of 34 滋 m using an arrayer (Beecher, USA).The tissue array with HE staining was examined by pathologists.Mouse antihuman Ezrin monoclonal antibody was produced by Neomarker Co., mouse antihuman Ecadherin monoclonal antibody by Santa Cruz Co., immunohistochemistry kit and diaminobenzidine (DAB) were purchased from Zymed Co. (USA).Immunohistochemical staining was performed according to the instructions of the kit.The sections were placed in an oven at 70益 overnight, dewaxed and hydrated, then put into 3% H 2 O 2 and incubated for 15 min to deactivate endogenous peroxidase, washed with PBS (pH 7.4) and put into citrate buffer (pH 6.0).Microwave or high pressure method was used to retrieve antigen for 15 min.The sections Expression and clinical significance of Ezrin and Ecadherin in esophageal squamous cell carcinoma