2016•International Journal of Farm SciencesRequires access
Spectrum of viable mutants in M2 generation of blackgram, Vigna mungo (L) Hepper through induced mutation
Loyavar Ramchander, N. Shunmugavalli, I MUTHUSWAMY
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Abstract
The present investigation was conducted during the Kharif season of 2013; seeds of black gram, Vingo mungo (L) Hepper variety VBN 4 and ACM 07002 were exposed to gamma rays doses at 200, 250, 300 and 350 Gy and EMS (ethyle methane sulphonate) doses at 20, 30 and 40 mM. In variety VBN 4 a total of 40 mutants were observed in the gamma rays and 17 mutants in the EMS treated plants. The more number of mutants was recorded in gamma ray and less number of mutants was recorded in EMS. Among the four doses of gamma rays treatment and three doses of EMS, the dose 350 Gy in gamma ray mutagen and 40 mM in EMS mutagen registered highest frequency. In ACM 07002 a total of 28 mutants were observed in the gamma rays and 16 mutants in the EMS treated plants. The more number of mutants was recorded in gamma ray and less number of mutants was recorded in EMS. Among the four doses of gamma rays treatment and three doses of EMS the dose 300 Gy in gamma ray mutagen and 40 mM in EMS mutagen registered highest frequency.