2016•Data in BriefOpen access

Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

Mohan C. Manjegowda, Paridhi Singhal Gupta, Anil Mukund Limaye

Abstract

Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines.

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Available abstract

Key concepts: Polyclonal antibodies, Antiserum, Blot, Molecular biology, Antibody, GPER, Peptide, Affinity chromatography