2016•International Journal of Pharmacy and Pharmaceutical SciencesOpen access

PHYTOCHEMICAL SCREENING AND COMPARISON OF ANTIOXIDANT ACTIVITY OF WATER AND ETHANOL EXTRACT PROPOLIS FROM MALAYSIA

Umar Usman, Ainul Bahiyah Abu Bakar, Mahaneem Mohamed

Abstract

Objective: In an attempt to compare extraction yield, phytochemical screening, radical scavenging activity, and total phenolic and flavonoid contents of water extract propolis (WEP) and ethanol extract propolis (EEP) from Malaysia. Methods: Raw propolis was extracted using distilled water and ethanol at various concentrations (10-100%) and the extraction yields were presented in percentage. The phytochemical screening, radical scavenging activity, total phenols using Folin-Ciocalteu method and flavonoid using aluminum chloride method of WEP and EEP were determined. Results: Malaysian propolis extracted using 70% ethanol produced the highest extraction yield and had significantly higher radical scavenging activity as well as total phenolic and flavonoid contents compared to WEP. Conclusion: Malaysian propolis extract using 70% ethanol is potential to be developed as a good antioxidant agent. Keywords: Antioxidant, Extractions, Malaysian, Propolis, Phytochemical screening

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Key concepts: Propolis, Phytochemical, Flavonoid, Chemistry, Extraction (chemistry), Phenols, Ethanol, Antioxidant