A COMPARATIVE IMMUNOHISTO CHEMICAL DIAGNOSIS OF HODGKIN’S LYMPHOMA WITH CONVENTIONAL METHOD

Introduction Hodgkin�s lymphoma is a malignant neoplasm of B lymphocytes and less common T lymphocytes that morphologically can be mistaken with several benign and malignant diseases. These diseases have different course, treatments and prognosis from Hodgkin�s lymphoma. Immunohistochemical staining is very useful that will aid in separating Hodgkin�s Lymphoma from a series of benign and malignant lookalikes. Materials and Methods We Randomly selected 53 cases with primary diagnosis of Hodgkin�s lymphoma in pathology laboratory of Imam Reza Hospital from 1374-1384. to confirm or rule out of the primary diagnosis, microscopic evaluation and Immunohistochemical staining were done. Results Overall in 47 cases (88.7), Hodgkin�s lymphoma was confirmed and 6 cases (11.3) were non hodgkin lymphoma.A total 5 cases were T cell Rich large B cell lymphoma and 1 case was anaplasic large cell lymphoma probably. In Hodgkin�s lymphoma cases, the mean age was 40 years. Except for nodular sclerosis other types were more frequent in males. (M/F = 2.5).The commonest type was mixed cellularity (39 cases) (54.7). Conclusion Misdiagnosis of Hodgkin�s lymphoma without Immunohistochemical staining was 11.3. Because of significant difference between treatment and prognosis of Hodgkin�s lymphoma and other morphologically similar diseases, immunohistochemical staining particulary in lymphocytic depletion and nodular lymphocytic predominant Hodgkin�s lymphoma, is necessary and recommended.