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High level expression of Bacillus subtilis B2 Xylanase gene in Pichia pastoris

Shuangyan Han, Xiaoqiong Lin, Xi Zhang, Ying Chih Lin

Abstract

Xylanase is one of the important enzymes widely applied in food industries. The gene of Bacillus subtilis B2 xylanase was separated and cloned into the secreted expression vector pPIC9K to construct a recombinant plasmid pPIC9K-XYN. The plasmid was linearized and transformed into Pichia pastoris GS115 and Pichia pastoris recombinant strain GS115/pPIC9K-XYN was obtained. The xylanase activity of the culture supernatant of recombinant strain was up to 1542 U/mL towards beenchwood xylan. The optimal temperature and pH for the recombinant xylanase was 50℃ and 6.0, respectively. Besides, the recombinant xylanase showed good thermal stability and wide adaptability to pH.

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Key concepts: Xylanase, Pichia pastoris, Recombinant DNA, Bacillus subtilis, Chemistry, Plasmid, Microbiology, Gene