The regulation of SWI/SNF chromatin remodeling complexes on human genes under heat shock and IFN‐gamma treatment

The ATP dependent chromatin remodeling complex can repost nucleosomes on the genome DNA, which is crucial for the regulation of human genes. The ATPase subunits of the human SWI/SNF chromatin remodeling complexes induce ATP release for chromatin remodeling at the promoter of its target genes either independently or in coordination. Here, we explore how an IFN‐γ activating sequences in the promoter can mediate chromatin remodeling and enhance gene activation in human leukemic cells under non‐lethal heat shock or IFN‐γ treatment. Our data were obtained mainly from real‐time RT‐PCR, chromatin immunoprecipitation, promoter activity assay, endonuclease accessibility, immunoprecipitation and domain‐specific interactions. We show a substitution of one SWI/SNF chromatin remodeling complex by its counterpart in the promoter of the genes is a requisite in the activation of hsp90α and two immune genes under heat shock. However, a similar change under IFN‐γ treatment in the SWI/SNF chromatin remodeling complexes to activate a gene occurs only on certain specific genes and cell types. We present a novel example showing the similarity and difference at the chromatin level in regulating activation of human genes under heat shock and IFNγ induction. This work is funded by NSFC #30721063 & #90408007