2010•Linye kexue yanjiuRequires access

Analysis of Populations Genetic Diversity of Qiongzhuea tumidinoda Using AFLP Markers

Ru Guangxin, Jin-Ling Yuan, Duo Zhang, Guo G

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Abstract

Amplified fragment length polymorphism(AFLP)was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers,Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic(97.20%).PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%;the number of alleles were 1.898 6 and 1.919 5;the effective number of alleles were 1.585 0 and 1.568 3;the average genetic identities within 6 populations was 0.332 1;the average genetic identities within the 2 populations was 0.394 9;Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.

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Amplified fragment length polymorphism(AFLP)was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers,Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic(97.20%).PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%;the number of alleles were 1.898 6 and 1.919 5;the effective number of alleles were 1.585 0 and 1.568 3;the average genetic identities within 6 populations was 0.332 1;the average genetic identities within the 2 populations was 0.394 9;Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.

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Available abstract

Amplified fragment length polymorphism(AFLP)was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers,Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic(97.20%).PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%;the number of alleles were 1.898 6 and 1.919 5;the effective number of alleles were 1.585 0 and 1.568 3;the average genetic identities within 6 populations was 0.332 1;the average genetic identities within the 2 populations was 0.394 9;Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.

Key concepts: Amplified fragment length polymorphism, Biology, Genetic diversity, UPGMA, Genetics, Genetic distance, Allele, Genetic variation

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