2010•Zhongguo yufang shouyi xuebaoRequires access
Detection of toxigenic Pasteurella multocida with a colony diplex PCR assay.
Li WeiJie, Zhao Yun, Du XinBo, Kai Kang, Min Chen
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Abstract
A duplex PCR assay was developed using two sets of specific primers derived from the KMT1 gene and toxA gene of Pasteurella multocida in order to differentiate toxigenic and nontoxigenic P. multocida at the same time. The assay could specifically amplify both 460 bp and 1 854 bp DNA products from thee toxigenic P. multocida C51-6,but not from nontoxigenic P. multocida,E. coli,Actinobacillus pleuropneumoniae,Streptococcus suis,Bordetella bronchiseptica,Haemophilus parasuis and Salmonella enterica subsp. It is highly sensitive and could detect as little as 450 CFU bacteria. The assay was verifiable by Guinea pig skin test and mouse lethal test.