Effect of N-acetylcysteine on expression of NF-κ B and ICAM-1 in rats with hepatic ischemia/reperfusion injury.

Objective:To investigate the expression of NF-κB and ICAM-1mechanisms in the Hepatic Ischemia/Reperfusion injury of rats,and the protective mechanisms of NAC.Methods:Forty-five male SD rats were randomly divided into three groups:sham operation group(Sham group n=5);Ischemia-Reperfusion group(I/R group n=20):Anaesthetized rats were subjected to 60min of sus-tained occlusion of the blood for left and middle liver followed by 1 hours,3 hours,6 hours and 12 hours of reperfusion respectively;(3)NAC group(N group n=20):NAC dissolved in the saline was injected from dorsal vein of penis,then rats were dealled as the I/R group 20min later.At the end of reperfusion,liver tissues samples were obtained for detection the experssion of NF-κB and ICAM-1 by meth-ods of western-blot and immunohistochemistry.Results:After reperfusion1h,3h,6h and 12h later,the expression of NF-κB in I/R and NAC groups were higher than Sham group(P0.01),and reached the peak after reperfusion 3h;The expression of ICAM-1 were all high-er than Sham group(P0.01),and reached the peak at reperfusion 6h.At 1h,3h,6h after reperfusion of NAC group,the expression of NF-κB and ICAM-1 were lower than the same time of I/R group(P0.05).At 12h after reperfusion,the the expression of NF-κB and ICAM-1 seem lower than the the same time of I/R group,but there was no difference between the two groups(p0.05).Conclusion:The expression of NF-κB and ICAM-1 increased obviously in Hepatic Ischemia/Reperfusion of rat,NAC can attenuate Hepatic Is-chem/Reperfusion injury by inhibiting the activation and expression of NF-κB and reduce the expression of ICAM-1.