2012•Unpublished venueRequires access
ESTABELECIMENTO IN VITRO DE CLONES HÍBRIDOS DE Eucalyptus globulus IN VITRO ESTABLISHMENT OF Eucalyptus globulus HYBRID CLONES
Wagner Campos Otoni, Elizabete Keiko Takahashi, Lucas Amaral de Melo
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Abstract
The present study aimed to evaluate the behavior of Eucalyptus grandis x Eucalyptus globulus and Eucalyptus urophylla x Eucalyptus globulus clones and the effect of different in vitro insertions (at 30, 90 and 150 days after pruning the donor plants) in the in vitro establishment phase. The mini-stump plants, explant providers for the in vitro introductions, were cultivated under an indoor semi-hydroponic clonal mini-hedging. Nodal segment explants of 21 Eucalyptus urophylla x Eucalyptus globulus clones and eight Eucalyptus grandis x Eucalyptus globulus clones were harvested, disinfected and inoculated in an MS culture medium, supplemented with 0.5 mg L -1 of BAP and 0.1 mg L -1 of NAA. After 30 days in culture, evaluations were made and it was concluded that there were variations among the clones, as well as in vitro