2015AgroLife Scientific JournalOpen access

Ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp. and Pseudomonas putida in bioremediation of waste water in cisirung waste water treatment plant

Ratu Safitri, Bambang Priadie, Mia Miranti, Alia Astuti

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Abstract

This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater origin Cisirung WWTP. This study uses an experimental method completely randomized design (CRD), which consists of two treatment factors (8x8 factorial design). The first factor is a consortium of bacteria (K), consisting of 8 level factors (k1, k2, k3, k4, k5, k6, k7, and k8). The second factor is the time (T), consisting of a 7 level factors (t0, t1, t2, t3, t4, t5, t6, and t7). Test parameters consist of BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), TSS (Total Suspended Solid), Ammonia and Population of Microbes during bioremediation. Data were analyzed by ANOVA, followed by Duncan test. The results of this study showed that the consortium of Bacillus pumilus, Bacillus subtilis, Bacillus coagulans, Nitrosomonas sp., and Pseudomonas putida with inoculum concentration of 5% (k6) is a consortium of the most effective in reducing BOD 71.93%, 64.30% COD, TSS 94.85%, and 88.58% of ammonia.

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This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater origin Cisirung WWTP. This study uses an experimental method completely randomized design (CRD), which consists of two treatment factors (8x8 factorial design). The first factor is a consortium of bacteria (K), consisting of 8 level factors (k1, k2, k3, k4, k5, k6, k7, and k8). The second factor is the time (T), consisting of a 7 level factors (t0, t1, t2, t3, t4, t5, t6, and t7). Test parameters consist of BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), TSS (Total Suspended Solid), Ammonia and Population of Microbes during bioremediation. Data were analyzed by ANOVA, followed by Duncan test. The results of this study showed that the consortium of Bacillus pumilus, Bacillus subtilis, Bacillus coagulans, Nitrosomonas sp., and Pseudomonas putida with inoculum concentration of 5% (k6) is a consortium of the most effective in reducing BOD 71.93%, 64.30% COD, TSS 94.85%, and 88.58% of ammonia.

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Available abstract

This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater origin Cisirung WWTP. This study uses an experimental method completely randomized design (CRD), which consists of two treatment factors (8x8 factorial design). The first factor is a consortium of bacteria (K), consisting of 8 level factors (k1, k2, k3, k4, k5, k6, k7, and k8). The second factor is the time (T), consisting of a 7 level factors (t0, t1, t2, t3, t4, t5, t6, and t7). Test parameters consist of BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), TSS (Total Suspended Solid), Ammonia and Population of Microbes during bioremediation. Data were analyzed by ANOVA, followed by Duncan test. The results of this study showed that the consortium of Bacillus pumilus, Bacillus subtilis, Bacillus coagulans, Nitrosomonas sp., and Pseudomonas putida with inoculum concentration of 5% (k6) is a consortium of the most effective in reducing BOD 71.93%, 64.30% COD, TSS 94.85%, and 88.58% of ammonia.

Key concepts: Bacillus pumilus, Pseudomonas putida, Bacillus coagulans, Bacillus licheniformis, Bacillus subtilis, Biochemical oxygen demand, Chemical oxygen demand, Bioremediation

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Ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp. and Pseudomonas putida in bioremediation of waste water in cisirung waste water treatment plant — Research Paper | ScholarLens