Laboratory surveillance of Vibrio parahaemolyticus strains from Shenzhen in 2007.

[Objective]To analyze the distribution of serotype, virulence gene and genetic relationship among Vibrio parahaemolyticus strains from Shenzhen in 2007.[Methods] Serotype was determined for 45 isolates.The tdh of virulence gene were assayed by real-time PCR.45 isolates were analyzed by pulsed-field gel electrophoresis with the restriction enzyme NotI.[Results] There were 5 serotypes.27 strains were serotype O3:K6.All strains were positive using real-time PCR.Most of serotype O3:K6 strains belonged to 3 distinctive PFGE patterns.Most of other serotype strains were identical PFGE patterns respectively.[Conclusion] The serotype O3:K6 is main type in Vibrio parahaemolyticus.Some strains have common sources.Surveillance based on laboratory will contribute to active surveillance and alarming for diarrhea of Vibrio parahaemolyticus.