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1997•Journal of Applied Animal ResearchOpen access

Restriction Mapping of HindIII Fragment ‘J’ of Bovine Herpesvirus-1 DNA Cloned in Opposite Orientation

R. S. Kataria, A. Rai

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Abstract

Kataria, R.S. and Rai, A. 1997. Restriction mapping of HindIII fragment ‘J’ of bovine herpesvirus-1 DNA cloned in opposite orientation. J. Appl. Anim. Res., 11: 183–188. Cloning of HindIII fragments of bovine herpesvirus-1 DNA in pUC9 plasmid vector yielded recombinants carrying ‘J’ fragment cloned in two opposite orientations in different clones. It could be revealed by digesting recombinant plasmids with restriction enzymes SmaI and PstI. Recombinant plasmids were also characterized by digesting with restriction enzymes HaeIII and EcoRI.

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Kataria, R.S. and Rai, A. 1997. Restriction mapping of HindIII fragment ‘J’ of bovine herpesvirus-1 DNA cloned in opposite orientation. J. Appl. Anim. Res., 11: 183–188. Cloning of HindIII fragments of bovine herpesvirus-1 DNA in pUC9 plasmid vector yielded recombinants carrying ‘J’ fragment cloned in two opposite orientations in different clones. It could be revealed by digesting recombinant plasmids with restriction enzymes SmaI and PstI. Recombinant plasmids were also characterized by digesting with restriction enzymes HaeIII and EcoRI.

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Available abstract

Kataria, R.S. and Rai, A. 1997. Restriction mapping of HindIII fragment ‘J’ of bovine herpesvirus-1 DNA cloned in opposite orientation. J. Appl. Anim. Res., 11: 183–188. Cloning of HindIII fragments of bovine herpesvirus-1 DNA in pUC9 plasmid vector yielded recombinants carrying ‘J’ fragment cloned in two opposite orientations in different clones. It could be revealed by digesting recombinant plasmids with restriction enzymes SmaI and PstI. Recombinant plasmids were also characterized by digesting with restriction enzymes HaeIII and EcoRI.

Key concepts: HindIII, EcoRI, Restriction enzyme, Recombinant DNA, Plasmid, PstI, HaeIII, Molecular biology

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